To solve this problem, we need to analyze each statement related to mitosis and identify the incorrect one. Let's examine each option:$\newline \bullet$Option 1: All the chromosomes lie at the equator at metaphase.$\newline$ - During metaphase, chromosomes align at the metaphase plate (equator) of the cell.$\newline$ - This statement is correct.$\newline \bullet$Option 2: Spindle fibres attach to the centromere of chromosomes.$\newline$ - Spindle fibres actually attach to the kinetochores, which are protein structures on the centromeres.$\newline$ - This statement is incorrect because it oversimplifies the attachment process.$\newline \bullet$Option 3: Chromosomes decondense at telophase.$\newline$ - During telophase, chromosomes begin to decondense back into chromatin.$\newline$ - This statement is correct.$\newline \bullet$Option 4: Splitting of centromere occurs at anaphase.$\newline$ - During anaphase, the centromeres split, allowing sister chromatids to separate and move to opposite poles.$\newline$ - This statement is correct.$\newline$Therefore, the incorrect statement is Option 2.