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Did you know?
Diamonds and graphite are both made of pure carbon — just arranged differently.
Did you know?
Diamonds and graphite are both made of pure carbon — just arranged differently.
Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence: A. Insertion of recombinant DNA into the host cell. B. Cutting of DNA at specific location by restriction enzyme. C. Isolation of desired DNA fragment. D. Amplification of gene of interest using PCR. Choose the correct answer from the options given below:
B, C, D, A
C, A, B, D
C, B, D, A
B, D, A, C
To solve this problem, we need to arrange the steps in the formation of recombinant DNA in the correct sequence. Let's analyze each step:C. Isolation of desired DNA fragment: - The first step in forming recombinant DNA is to isolate the DNA fragment that contains the gene of interest.B. Cutting of DNA at specific location by restriction enzyme: - Once the desired DNA fragment is isolated, restriction enzymes are used to cut the DNA at specific sites to facilitate the insertion of the gene of interest.D. Amplification of gene of interest using PCR:
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