To solve this problem, we need to analyze each option regarding enzyme action:$\newline \bullet$Option 1: Substrate binds with enzyme at its active site.$\newline$ - This statement is correct. The active site of an enzyme is the region where the substrate binds and undergoes a chemical reaction.$\newline \bullet$Option 2: Addition of lot of succinate does not reverse the inhibition of succinic dehydrogenase by malonate.$\newline$ - This statement is incorrect. Malonate is a competitive inhibitor of succinic dehydrogenase.$\newline$ - In competitive inhibition, increasing the concentration of the substrate (succinate) can overcome the inhibition.$\newline \bullet$Option 3: A non-competitive inhibitor binds the enzyme at a site distinct from that which binds the substrate.$\newline$ - This statement is correct. Non-competitive inhibitors bind to an allosteric site, which is different from the active site.$\newline$ - This binding changes the enzyme's shape and function, reducing its activity regardless of substrate concentration.$\newline \bullet$Option 4: Malonate is a competitive inhibitor of succinic dehydrogenase.$\newline$ - This statement is correct. Malonate competes with succinate for the active site of succinic dehydrogenase.$\newline$Therefore, the option that is not correct with respect to enzyme action is Option 2.