To solve this problem, we need to determine which option correctly describes the cryopreservation of gametes of threatened species in a viable fertile condition.$\newline$Let's analyze each option:$\newline \bullet$Option 1: In situ conservation of biodiversity refers to the conservation of species in their natural habitats. Cryopreservation does not occur in natural habitats, so this option is incorrect.$\newline \bullet$Option 2: Advanced ex situ conservation of biodiversity involves the preservation of components of biological diversity outside their natural habitats. Cryopreservation is a method of ex situ conservation, making this option correct.$\newline \bullet$Option 3: In situ conservation by sacred groves involves protecting species within their natural environment, often in areas considered sacred. This does not involve cryopreservation, so this option is incorrect.$\newline \bullet$Option 4: In situ cryoconservation of biodiversity is not a recognized method, as cryopreservation is inherently an ex situ technique. Therefore, this option is incorrect.$\newline$Based on the analysis, the correct option is Option 2: Advanced ex situ conservation of biodiversity.