Plasmid pBR322 has PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for Ī²-galactosidase production and the recombinant plasmid is introduced in an E.coli strain:
medium
Biotechnology Principles and Processes
2021
biology
It will not be able to confer ampicillin resistance to the host cell.
The transformed cells will have the ability to resist ampicillin as well as produce Ī²-galactosidase.
It will lead to lysis of host cell.
It will be able to produce a novel protein with dual ability.
Explanation
To solve this problem, we need to understand the implications of inserting a gene into the plasmid pBR322 using the PstI restriction enzyme site.Let's analyze the situation:āPlasmid pBR322 contains an ampicillin resistance gene, denoted as ampR.āThe PstI restriction enzyme cuts within the ampR
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