To solve this problem, we need to identify the correct sequence of steps in a PCR (Polymerase Chain Reaction). Let's analyze each step:$\newline \bullet$Denaturation:$\newline$ - This is the first step in PCR where the double-stranded DNA is heated to around 94-98°C.$\newline$ - The high temperature causes the hydrogen bonds between the DNA strands to break, resulting in two single strands.$\newline \bullet$Annealing:$\newline$ - In this step, the temperature is lowered to around 50-65°C.$\newline$ - This allows primers to bind or anneal to their complementary sequences on the single-stranded DNA.$\newline \bullet$Extension:$\newline$ - The temperature is then raised to around 72°C, which is the optimal temperature for DNA polymerase.$\newline$ - DNA polymerase extends the primers by adding nucleotides to synthesize the new DNA strand.$\newline$Now, let's determine the correct sequence of steps:$\newline$The correct sequence is: Denaturation, Annealing, Extension.$\newline$This corresponds to Option 1: Denaturation, Annealing, Extension.$\newline$Therefore, the correct option is Option 1.