To solve this problem, we need to determine under which condition there will be no change in the reading frame of the given mRNA sequence.$\newline$The original mRNA sequence is:$\newline 5'$AACAGCGGUGCUAUU$3'\newline$Let's analyze each option:$\newline \bullet$Option 1: Insertion of G at 5th position$\newline$ - The sequence becomes: 5'AACAGGCGGUGCUAUU3'$\newline$ - This insertion shifts the reading frame, causing a frameshift mutation.$\newline \bullet$Option 2: Deletion of G from 5th position$\newline$ - The sequence becomes: 5'AACACGGUGCUAUU3'$\newline$ - This deletion also shifts the reading frame, causing a frameshift mutation.$\newline \bullet$Option 3: Insertion of A and G at 4th and 5th positions respectively$\newline$ - The sequence becomes: 5'AACAAGCGGUGCUAUU3'$\newline$ - This insertion shifts the reading frame, causing a frameshift mutation.$\newline \bullet$Option 4: Deletion of GGU from 7th, 8th, and 9th positions$\newline$ - The sequence becomes: 5'AACACUCUAUU3'$\newline$ - This deletion removes a complete codon (GGU), so the reading frame is not altered.$\newline$Therefore, the correct option is Option 4, as it does not change the reading frame.